Sigma-Aldrich MINI67 PKH67 Green Fluorescent Cell Linker Mini Kit for General Cell Membrane Labeling Distributed for Phanos Technologies 1 Kit
Marka
Stok Kodu
LB.SA.MINI67-1KT
Kısa Bilgi
Synonym(s): Green PKH membrane mini labeling kit NACRES: NA.32
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Sigma-Aldrich MINI67 PKH67 Green Fluorescent Cell Linker Mini Kit for General Cell Membrane Labeling Distributed for Phanos Technologies 1 Kit |
|
Synonym(s): Green PKH membrane mini labeling kit
NACRES: NA.32 |
|
PROPERTIES
packaging pkg of 1 kit
manufacturer/tradename Distributed for Phanos Technologies
storage condition protect from light
technique(s) flow cytometry: suitable
fluorescence λex 490 nm; λem 502 nm (PKH67 dye)
application(s) cell analysis detection
detection method fluorometric
shipped in ambient
storage temp. room temp
DESCRIPTION
General description
PKH67 kit is for general cell membrane labeling. It has a longer aliphatic carbon tail than PKH1 and PKH2, two other green dyes previously described for in vitro and in vivo cell tracking. Based on the longer tail length, in-house studies have consistently shown reduced cell-cell transfer for PKH67 as compared to PKH2. Slow loss of fluorescence has been observed in in vivo studies using PKH1 and PKH2. PKH67 may exhibit similar properties since this behavior appears to be characteristic of green cell linker dyes, but not red cell linker dyes. Correlation of in vitro cell membrane retention with in vivo fluorescence half life in non-dividing cells predicts an in vivo fluorescence half life of 10-12 days for PKH67. Other green cell linker dyes with similar half lives have been used to monitor in vivo lymphocyte and macrophage trafficking over periods of 1-2 months, suggesting that PKH67 will also be useful for in vivo tracking studies of moderate length.
Application
PKH67 green fluorescent cell linker mini kit for general cell membrane labeling has been used to stain:
- exosomes[1]
- pericytes[2]
- UT-7/TPO cells[3]
This kit is for general cell membrane labeling. Slow loss of fluorescence has been observed in in vivo studies using PKH1 and PKH2. PKH67 may exhibit similar properties since this behavior appears to be characteristic of green cell linker dyes, but not red cell linker dyes.
Linkage
For additional technical details on PKH and CellVue® Fluorescent Cell Linker Dyes including an extensive bibliography, please visit here.
Legal Information
Distributed for Phanos Technologies, Inc. CellVue is a registered trademark of Phanos Technologies |
