Sigma-Aldrich P1435 Phosphatase, Acid from sweet potato ammonium sulfate suspension, ≥10.0 units/mg protein (modified Warburg-Christian) 2500 UNITS
| Sigma-Aldrich P1435 Phosphatase, Acid from sweet potato ammonium sulfate suspension, ≥10.0 units/mg protein (modified Warburg-Christian) 2500 UNITS |
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Synonym(s): (Orthophosphoric-monoester phosphohydrolase [acid optimum] )
CAS Number: 9001-77-8
Enzyme Commission number: 3.1.3.2 (BRENDA, IUBMB)
EC Number: 232-630-9
MDL number: MFCD00131847
NACRES: NA.54
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| PROPERTIES
form ammonium sulfate suspension
Quality Level 300
specific activity ≥10.0 units/mg protein (modified Warburg-Christian)
secondary activity ≤9.9 units/mg protein ATPase
mol wt 110 kDa
storage temp. 2-8 °C
DESCRIPTION
General description
Acid phosphatase from sweet potato is a phosphomonoesterase, which can appear in multiple molecular forms of similar molecular mass but with different isoelectric points.
Application
Potato acid phosphatase is used as a dephosphorylating reagent in thiochrome assays for the detection of thiamin in biological samples. In this assay, it was shown to be more effective than acid phosphatases from wheat germ or α-amylase. Sweet potato acid phosphatase has been used in a study as a pre-column enzyme reactor via a covalent bond with glutaraldehyde and aminopropyl controlled-pore glass. It has also been used in a study to investigate the use of tyrosine, histidine, and cysteine as ligands for Mn(III) in sweet potato phosphatase.
Biochem/physiol Actions
Acid phosphatases (APase) are a family of enzymes that non-specifically catalyze the hydrolysis of monoesters and anhydrides of phosphoric acid to produce inorganic phosphate at an optimum pH of 4 to 7 by the following reaction: APaseR-PO4 + H2O --------------- R-OH + HOPO3 2+Their function in the production, transport, and recycling of phosphate is critical for the metabolic and energy transduction processes of the cell. As a group, Apases may be as important as kinases in regulatory processes.
Unit Definition
One unit will hydrolyze 1.0 μmole of p-nitrophenyl phosphate per min at pH 4.8 at 37 °C.
Physical form
Suspension in 1.8 M (NH4)2SO4, 10 mM MgCl2, pH 5.3 |