Sigma-Aldrich P1644 Percoll® pH 8.5-9.5 (20 °C) 25 mL
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LB.SA.P1644-25ML
| Sigma-Aldrich P1644 Percoll® pH 8.5-9.5 (20 °C) 25 mL |
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MDL number: MFCD00131830
NACRES: NA.75
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PROPERTIES
sterility aseptically filled
Quality Level 200
color clear colorless to faint yellow
pH 8.5-9.5 (20 °C)
application(s) hematology histology
storage temp. 2-8 °C
DESCRIPTION
General description
Percoll® is a classic medium for density gradient centrifugation of cells, viruses, and subcellular particles. Percoll consists of colloidal silica particles of 15–30 nm diameter (23% w/w in water), which have been coated with polyvinylpyrrolidone (PVP). The PVP coating renders the product completely non-toxic and ideal for use with biological materials.
Application
Percoll consists of colloidal silica particles of 15-30 nm diameter (23% w/w in water) which have been coated with polyvinylpyrrolidone (PVP). It is used to establish non-toxic low viscosity, low osmolarity density gradients that can be used to isolate cells, organelles and viruses. It has been used to separate inner and outer membrane vesicles from E. coli. Percoll is isosmotic throughout the gradient, non-toxic, and is easily removed from the purified materials. Percoll® has been used in sperm preparation to study the energy requirements of bovine embryos.[74]
Legal Information
Percoll is a registered trademark of Cytiva |