Sigma-Aldrich P7170 Ponceau S solution BioReagent, suitable for electrophoresis, 0.1 % (w/v) in 5% acetic acid 1 L
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Sigma-Aldrich P7170 Ponceau S solution
BioReagent, suitable for electrophoresis, 0.1 % (w/v) in 5% acetic acid 1 L |
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Empirical Formula (Hill Notation): C22H12N4Na4O13S4
CAS Number: 6226-79-5
Molecular Weight: 760.57
MDL number: MFCD00003892
PubChem Substance ID: 24898827
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PROPERTIES
product line BioReagent
Quality Level 200
form liquid
concentration 0.1 % (w/v) in 5% acetic acid
density 1.01 g/cm3 at 20 °C
suitability suitable for electrophoresis
application(s)
diagnostic assay manufacturing
hematology
histology
storage temp. room temp
SMILES string [Na+].[Na+].[Na+].[Na+].Oc1c(\N=N\c2ccc(cc2S([O-])(=O)=O)\N=N\c3ccc(cc3)S([O-])(=O)=O)c4ccc(cc4cc1S([O-])(=O)=O)S([O-])(=O)=O
InChI 1S/C22H16N4O13S4.4Na/c27-22-20(43(37,38)39)10-12-9-16(41(31,32)33)6-7-17(12)21(22)26-25-18-8-3-14(11-19(18)42(34,35)36)24-23-13-1-4-15(5-2-13)40(28,29)30;;;;/h1-11,27H,(H,28,29,30)(H,31,32,33)(H,34,35,36)(H,37,38,39);;;;/q;4*+1/p-4/b24-23+,26-25+;;;;
InChI key KQHKSGRIBYJYFX-WNDMICSESA-J
DESCRIPTION
General description
Ponceau S is the sodium salt of a diazo dye. Ponceau S solution is a suitable reagent for use in electrophoresis studies. The Ponceau S stain comes ready-to-use and is designed for rapid (5 min) staining of protein bands on nitrocellulose or PVDF membranes (Western blots) and also for staining protein on cellulose acetate membranes. It may be employed as stain for the purified fibrinolytic enzyme (CK) for the analysis of N-terminal amino acid sequence of the first 14 residues of CK by a protein sequencer. Ponceau S staining on nitrocellulose and PVDF is reversible by washing the stained membrane with 0.1M NaOH for 1 minute. The stained and destained membrane can then be immunologically detected (Western blotting). A product information sheet is supplied which includes the staining procedure for cellulose acetate, nitrocellulose, and PVDF.
Application Ponceau S solution has been used for membrane staining (Western blotting).
Packaging 1 L in poly bottle |