Sigma-Aldrich R7876 Ribonucleic acid, transfer from wheat germ Type V, 15-19 units/mg solid 2500 Units
Marka
Stok Kodu
LB.SA.R7876-2.5KU
Kısa Bilgi
Synonym(s): Transfer RNA, tRNA CAS Number: 9014-25-9 MDL number: MFCD00132200 NACRES: NA.55
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Sigma-Aldrich R7876 Ribonucleic acid, transfer from wheat germ |
|
Synonym(s): Transfer RNA, tRNA
CAS Number: 9014-25-9
MDL number: MFCD00132200
NACRES: NA.55 |
|
PROPERTIES
grade for molecular biology
Quality Level 300
type Type V
form lyophilized powder
specific activity 15-19 units/mg solid
storage condition desiccated
solubility H2O: ~2 mg/mL, clear to hazy
storage temp. 2-8°C
DESCRIPTION
General description
Wheat germ tRNA contains many different specific tRNA molecules.
Application
Crude transfer RNA (tRNA), purified from wheat germ, contains many specific tRNA molecules that can be separated by different processes. Experiments for detailed investigation of structure and function using in vitro translation systems often require highly purified tRNA, which can be achieved by combinations of chromatographic separations and reversible biochemical modification of the tRNA. Wheat germ tRNA has been used for the purification of RNAse Z (tRNA 3′- processing endonucleases) from wheat germ. The purification included six steps with the most efficient one using an affinity column made of wheat germ tRNA, which the RNAse Z bound tightly. Suitable for use as a carrier in nucleic acid purification and precipitation.
Packaging
500 units in poly bottle
2500, 10000 units in glass bottle
Components
tRNA is provided as a lyophilized powder that is soluble in water at a concentration of 2 mg/ml.
Unit Definition
One unit will yield an absorbance of 1.0 at 260 nm in 1.0 mL of water (1 cm light path). |
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