Sigma-Aldrich R8758 RPMI-1640 Medium With L-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture 500 mL
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LB.SA.R8758-500ML
| Sigma-Aldrich R8758 RPMI-1640 Medium With L-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture 500 mL |
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Synonym(s): Roswell Park Memorial Institute 1640 medium
MDL number: MFCD00217820
NACRES: NA.75
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PROPERTIES
Quality Level 400
sterility sterile-filtered
form liquid
technique(s) cell culture | mammalian: suitable
impurities endotoxin, tested
components
sodium pyruvate: no
L-glutamine: yes
phenol red: yes
HEPES: no
NaHCO3: yes
shipped in ambient
storage temp. 2-8 °C
DESCRIPTION
General description
RPMI 1640 Medium was developed at Roswell Park Memorial Institute in 1966 by Moore and his co-workers. A modification of McCoy′s 5A Medium, it was formulated to support lymphoblastoid cells in suspension culture, but it has since been shown to support a wide variety of cells that are anchorage dependent. RPMI 1640 Medium was developed at Roswell Park Memorial Institute in 1966 by Moore and his co-workers. A modification of McCoy′s 5A Medium, it was formulated to support lymphoblastoid cells in suspension culture, but it has since been shown to support a wide variety of cells that are anchorage-dependent. Originally intended to be used with a serum supplement, RPMI 1640 has been shown to support several cell lines in the absence of serum. It has also been widely used in fusion protocols and in the growth of hybrid cells. This medium is suitable for culturing human normal and neoplastic leukocytes.
Application
RPMI-1640 Medium has been used for maintaining cell line medium and serves as a medium for different cells. |
