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Sigma Aldrich S0389 Sucrose for molecular biology, ≥99.5% (GC) 1 kg

Stok Kodu
LB.SA.S0389-1KG
Kısa Bilgi
Synonym(s): α-D-Glc-(1→2)-β-D-Fru, α-D-Glucopyranosyl β-D-fructofuranoside, β-D-Fructofuranosyl-α-D-glucopyranoside, D(+)-Saccharose, Sugar Empirical Formula (Hill Notation): C12H22O11 CAS Number: 57-50-1 Molecular Weight: 342.30 Beilstein: 90825 EC Number: 200-334-9 MDL number: MFCD00006626 PubChem Substance ID: 24899423 NACRES: NA.31
Ön Siparişli Ürün
Sigma Aldrich S0389 Sucrose for molecular biology, ≥99.5% (GC) 1 kg
Synonym(s): α-D-Glc-(1→2)-β-D-Fru, α-D-Glucopyranosyl β-D-fructofuranoside, β-D-Fructofuranosyl-α-D-glucopyranoside, D(+)-Saccharose, Sugar
Empirical Formula (Hill Notation):
C12H22O11
CAS Number:
57-50-1
Molecular Weight:
342.30
Beilstein:
90825
EC Number:
200-334-9
MDL number:
MFCD00006626
PubChem Substance ID:
24899423
NACRES:
NA.31
Sigma Aldrich S0389 Sucrose for molecular biology, ≥99.5% (GC)        
PROPERTIES
biological source sugar cane
Quality Level
300
grade
for molecular biology
Assay
≥99.5% (GC)
form
crystals
technique(s)
HPLC: suitable
cryopreservation: suitable
electron microscopy: suitable
enzyme immunoassay: suitable
gas chromatography (GC): suitable
immunohistochemistry: suitable
impurities ≤0.1% free glucose
color
white
useful pH range
5.5-7 (25 °C, 342 g/L)
mp
185-187 °C (lit.)
solubility
H2O: soluble 50 g + 50 mL
cation traces
heavy metals (as Pb): ≤5 ppm
absorption
≤0.15 at 280 at 50%
≤0.20 at 260 at 50%
suitability
suitable for molecular biology
application(s)
agriculture 
life science and biopharma
metabolomics
sample preservation
foreign activity
DNase, RNase, protease, none detected
storage temp.
room temp
SMILES string
OC[C@H]1O[C@H](O[C@]2(CO)O[C@H](CO)[C@@H](O)[C@@H]2O)[C@H](O)[C@@H](O)[C@@H]1O
InChI 1S/C12H22O11/c13-1-4-6(16)8(18)9(19)11(21-4)23-12(3-15)10(20)7(17)5(2-14)22-12/h4-11,13-20H,1-3H2/t4-,5-,6-,7-,8+,9-,10+,11-,12+/m1/s1
InChI key
CZMRCDWAGMRECN-UGDNZRGBSA-N


DESCRIPTION

General description
Sucrose, a disaccharide composed of glucose and fructose, serves as an energy source in various biological processes. In cell biology, sucrose acts as a cryoprotectant, preserving cell viability during freezing and thawing. It is also added as a supplement in culture media, aiding in maintaining osmotic balance and enhancing cell survival.In molecular biology, sucrose is crucial in gradient solutions for DNA and RNA purification and in density centrifugation for organelle separation.
Application
Sucrose has been used:
- to perform cryoprotection for kidney tissues
- as a component in the solid germination medium to germinate pollen grains
- in the triphenyl-tetrazolium chloride (TTC) test to check the viability of pollen grains
- in the cryoprotection of fixed brain tissues for immunohistochemistry
Biochem/physiol Actions
Sucrose is used as a transport and storage molecule in most plants due to its limited chemical reactivity. It can be used to prepare density gradients for the separation of cell/organelle. Sucrose can be used in the plant, insect, and bacterial culture media as a supplement. In addition, sucrose can also be used in several enzymatic assays.
Features and Benefits
- Ideal for Molecular Biology, Cell Biology and Biochemical research
- Tested to confirm low levels of heavy metal contamination, ensuring suitability for various applications
- Tested for DNase, RNase, and proteases
- Molecular biology grade sucrose, sourced from sugar cane

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Sigma Aldrich S0389 Sucrose for molecular biology, ≥99.5% (GC)
Sigma Aldrich S0389 Sucrose for molecular biology, ≥99.5% (GC) 1 kg Empirical Formula (Hill Notation): C12H22O11, CAS Number: 57-50-1, Molecular Weight: 342.30 Beilstein: 90825 EC Number: 200-334-9 MDL number: MFCD00006626 LB.SA.S0389-1KG
Sigma Aldrich S0389 Sucrose for molecular biology, ≥99.5% (GC) 1 kg

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