Sigma Aldrich S0389 Sucrose for molecular biology, ≥99.5% (GC) 5 kg
Marka
Stok Kodu
LB.SA.S0389-5KG
Kısa Bilgi
Synonym(s): α-D-Glc-(1→2)-β-D-Fru, α-D-Glucopyranosyl β-D-fructofuranoside, β-D-Fructofuranosyl-α-D-glucopyranoside, D(+)-Saccharose, Sugar Empirical Formula (Hill Notation): C12H22O11 CAS Number: 57-50-1 Molecular Weight: 342.30 Beilstein: 90825 EC Number: 200-334-9 MDL number: MFCD00006626 PubChem Substance ID: 24899423 NACRES: NA.31
| Sigma Aldrich S0389 Sucrose for molecular biology, ≥99.5% (GC) 5 kg |
| Synonym(s): α-D-Glc-(1→2)-β-D-Fru, α-D-Glucopyranosyl β-D-fructofuranoside, β-D-Fructofuranosyl-α-D-glucopyranoside, D(+)-Saccharose, Sugar Empirical Formula (Hill Notation): C12H22O11 CAS Number: 57-50-1 Molecular Weight: 342.30 Beilstein: 90825 EC Number: 200-334-9 MDL number: MFCD00006626 PubChem Substance ID: 24899423 NACRES: NA.31 |
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PROPERTIES
biological source sugar cane
Quality Level 300 grade for molecular biology Assay ≥99.5% (GC) form crystals technique(s) HPLC: suitable cryopreservation: suitable electron microscopy: suitable enzyme immunoassay: suitable gas chromatography (GC): suitable immunohistochemistry: suitable impurities ≤0.1% free glucose color white useful pH range 5.5-7 (25 °C, 342 g/L) mp 185-187 °C (lit.) solubility H2O: soluble 50 g + 50 mL cation traces heavy metals (as Pb): ≤5 ppm absorption ≤0.15 at 280 at 50% ≤0.20 at 260 at 50% suitability suitable for molecular biology application(s) agriculture life science and biopharma metabolomics sample preservation foreign activity DNase, RNase, protease, none detected storage temp. room temp SMILES string OC[C@H]1O[C@H](O[C@]2(CO)O[C@H](CO)[C@@H](O)[C@@H]2O)[C@H](O)[C@@H](O)[C@@H]1O InChI 1S/C12H22O11/c13-1-4-6(16)8(18)9(19)11(21-4)23-12(3-15)10(20)7(17)5(2-14)22-12/h4-11,13-20H,1-3H2/t4-,5-,6-,7-,8+,9-,10+,11-,12+/m1/s1 InChI key CZMRCDWAGMRECN-UGDNZRGBSA-N DESCRIPTION General description Sucrose, a disaccharide composed of glucose and fructose, serves as an energy source in various biological processes. In cell biology, sucrose acts as a cryoprotectant, preserving cell viability during freezing and thawing. It is also added as a supplement in culture media, aiding in maintaining osmotic balance and enhancing cell survival.In molecular biology, sucrose is crucial in gradient solutions for DNA and RNA purification and in density centrifugation for organelle separation. Application Sucrose has been used: - to perform cryoprotection for kidney tissues - as a component in the solid germination medium to germinate pollen grains - in the triphenyl-tetrazolium chloride (TTC) test to check the viability of pollen grains - in the cryoprotection of fixed brain tissues for immunohistochemistry Biochem/physiol Actions Sucrose is used as a transport and storage molecule in most plants due to its limited chemical reactivity. It can be used to prepare density gradients for the separation of cell/organelle. Sucrose can be used in the plant, insect, and bacterial culture media as a supplement. In addition, sucrose can also be used in several enzymatic assays. Features and Benefits - Ideal for Molecular Biology, Cell Biology and Biochemical research - Tested to confirm low levels of heavy metal contamination, ensuring suitability for various applications - Tested for DNase, RNase, and proteases - Molecular biology grade sucrose, sourced from sugar cane |
