Sigma-Aldrich S9430 SYBR® Green I nucleic acid gel stain 10,000 x in DMSO 1 mL
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Stok Kodu
LB.SA.S9430-1ML
Kısa Bilgi
Synonym(s): DNA stain, SYBR® green gel dye, safer gel stain CAS Number: 163795-75-3 MDL number: MFCD00284715 NACRES: NA.52
Bilgi
CAS Number: 163795-75-3
| Sigma-Aldrich S9430 SYBR® Green I nucleic acid gel stain 10,000 x in DMSO 0.5 mL |
| Synonym(s): DNA stain, SYBR® green gel dye, safer gel stain CAS Number: 163795-75-3 MDL number: MFCD00284715 NACRES: NA.52 |
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PROPERTIES
form solution
Quality Level 200 usage mL sufficient for 100 mini-gels greener alternative product characteristics Designing Safer Chemicals concentration 10,000 × in DMSO technique(s) PCR: suitable greener alternative category Aligned, storage temp. −20°C DESCRIPTION General description SYBR® Green I is a proprietary asymmetrical cyanine dye, which is used to detect nucleic acids. It consists of a N-alkylated benzothiazolium or benzoxazolium ring system, that is joined by a monomethine bridge to a pyridinium or quinolinium ring system.[1] SYBR Green I binds to the minor groove of dsDNA and is excited at a wavelength of 480 nm. It has a peak fluorescence emission of 520 nm.[2] Application SYBR® Green I nucleic acid gel stain has been used for: - the quantification of dsDNA[3] - to stain DNA in polymerase chain reaction (PCR)[4] - for comet assay technique[5] - to assess spermatozoon membrane integrity[6] - for visual inspection of DNA amplified by loop-mediated isothermal amplification (LAMP)[7] - as a fluorescent dye in flow cytometry[8] - real-time quantitative reverse transcription-polymerase chain reaction (qRT-PCR) for staining reverse-transcribed cDNA[8] Features and Benefits - An ultrasensitive stain for post-electrophoresis staining of dsDNA in agarose or polyacrylamide gels It can also detect ssDNA and RNA in denaturing agarose/formaldehyde and polyacrylamide/urea gels without any pre-washing steps - It is less mutagenic than ethidium bromide in Ames tests - It provides 50-100 times greater detection sensitivity than ethidium bromide for oligonucleotides - Useful for many applications with a limited amount of DNA - The binding of SYBR® Green I to DNA does not inhibit the activity of many common restriction endonucleases, including Hind III and EcoR I - Removal of this stain in-gel digestion and ligation techniques is not needed - SYBR Green I is a greener alternative product to ethidium bromide for staining Storage and Stability Store the product at –20 °C. The diluted Staining Solution may be stored, protected from light, either at 2–8 °C for several weeks or at room temperature for 3–4 days. Legal Information SYBR is a registered trademark of Life Technologies |
