Sigma-Aldrich T0377 Tricine ≥99% (titration) 1 kg
Marka
Stok Kodu
LB.SA.T0377-1KG
Kısa Bilgi
Synonym(s): N-[Tris(hydroxymethyl)methyl]glycine Linear Formula: (HOCH2)3CNHCH2CO2H CAS Number: 5704-04-1 Molecular Weight: 179.17 Beilstein: 1937804 EC Number: 227-193-6 MDL number: MFCD00004277 UNSPSC Code: 12161700 PubChem Substance ID: 24899884 NACRES: NA.25
Bilgi
CAS Number: 5704-04-1
| Sigma-Aldrich T0377 Tricine ≥99% (titration) |
| Synonym(s): N-[Tris(hydroxymethyl)methyl]glycine Linear Formula: (HOCH2)3CNHCH2CO2H CAS Number: 5704-04-1 Molecular Weight: 179.17 Beilstein: 1937804 EC Number: 227-193-6 MDL number: MFCD00004277 UNSPSC Code: 12161700 PubChem Substance ID: 24899884 NACRES: NA.25 |
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PROPERTIES
Quality Level 400
Assay ≥99% (titration) form crystalline powder useful pH range 7.4-8.8 pKa (25 °C) 8.1 mp 187 °C solubility water: 0.25 g/mL, clear, colorless application(s) diagnostic assay manufacturing SMILES string OCC(CO)(CO)NCC(O)=O InChI 1S/C6H13NO5/c8-2-6(3-9,4-10)7-1-5(11)12/h7-10H,1-4H2,(H,11,12) InChI key SEQKRHFRPICQDD-UHFFFAOYSA-N DESCRIPTION General description Tricine is the most frequently used electrophoresis buffer. It is also useful for the resuspension of cell pellets. Tricine functions as a trailing ion and aids the resolution of small proteins at lower acrylamide concentrations than in glycine-sodium dodecyl-sulfate polyacrylamide gel electrophoresis (SDS-PAGE) systems. It has a lower negative charge than glycine, which helps it migrate faster. Tricine has a high ionic strength that allows increased ion movement and less protein movement. This in turn leads to the separation of low molecular weight proteins in lower percent acrylamide gels.[1] Application Tricine has been used: - to prevent precipitation of salts during autoclaving of Emiliania huxleyi cultures[2] - as a component of buffer A for the homogenization of samples like Caenorhabditis elegans, Drosophila, and plants[3] - as a component of fresh assay buffer to measure serum melatonin by radioimmunoassay[4] Buffer component for separation of low molecular weight peptides |