Sigma-Aldrich T0665 holo-Transferrin human powder, BioReagent, suitable for cell culture, ≥97% 500 mg
Marka
Stok Kodu
LB.SA.T0665-500MG
Kısa Bilgi
Synonym(s): Siderophilin, iron-saturated CAS Number: 11096-37-0 EC Number: 234-318-8 MDL number: MFCD00131337 NACRES: NA.75
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Sigma-Aldrich T0665 holo-Transferrin human powder, BioReagent, suitable for cell culture, ≥97% 500 mg
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Synonym(s): Siderophilin, iron-saturated
CAS Number: 11096-37-0
EC Number: 234-318-8
MDL number: MFCD00131337
NACRES: NA.75
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PROPERTIES
biological source human plasma
Quality Level 200
description Iron-saturated
product line BioReagent
assay ≥97%
form powder
technique(s) cell culture | mammalian: suitable
impurities HIV and HBsAg, none detected endotoxin, tested
solubility H2O: 50 mg/mL
UniProt accession no. P02787
shipped in ambient
storage temp. 2-8°C
Gene Information human ... TF(7018)
DESCRIPTION
Application
Transferrin is the primary iron binding and cell delivery molecule present in serum. Serum-free cell culture systems require a delivery format for iron. Transferrin is the preferential delivery form of iron because cells process transferrin bound iron in a physiologically appropriate way through transferrin receptors on the cell surface. Human holo-transferrin is a high affinity transferrin that can be used with a wide range of cells types. Human holo-transferrin is loaded with iron prior to use and can be added directly to iron poor or iron-free cell culture media. holo-Transferrin human has been supplemented in Roswell Park Memorial Institute (RPMI) for proerythroblasts proliferation assay[1]
and also in culture media for inductively coupled plasma emission spectroscopy.[2]
Packaging
50, 100, 500 mg in poly bottle
1 g in poly bottle
Biochem/physiol Actions
Provides iron to cells in a physiologically stable and safe form. Preparation Note Heat treated at 60 °C (minimum) for a minimum of 10 hours.
Analysis Note
Iron content by ICP reported on a lot-specific basis. Purity by agarose gel electrophoresis.
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