Sigma-Aldrich T1503 Trizma® base ≥99.9% (titration), crystalline, primary standard, aminopeptidase substrate CAS No.: 77-86-1 500 gr
Marka
Stok Kodu
LB.SA.T1503-500G
Kısa Bilgi
Synonym(s): 2-Amino-2-(hydroxymethyl)-1,3-propanediol, THAM, Tris base, Tris(hydroxymethyl)aminomethane, Trometamol Linear Formula: NH2C(CH2OH)3 CAS Number: 77-86-1 Molecular Weight: 121.14 Beilstein: 741883 EC Number: 201-064-4 MDL number: MFCD00004679 UNSPSC Code: 12352104 PubChem Substance ID: 24899973 NACRES: NA.25
Bilgi
CAS Number: 77-86-1
| Sigma-Aldrich T1503 Trizma® base ≥99.9% (titration), crystalline, primary standard, aminopeptidase substrate |
| Synonym(s): 2-Amino-2-(hydroxymethyl)-1,3-propanediol, THAM, Tris base, Tris(hydroxymethyl)aminomethane, Trometamol Linear Formula: NH2C(CH2OH)3 CAS Number: 77-86-1 Molecular Weight: 121.14 Beilstein: 741883 EC Number: 201-064-4 MDL number: MFCD00004679 UNSPSC Code: 12352104 PubChem Substance ID: 24899973 NACRES: NA.25 |
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PROPERTIES
Product Name Trizma® base, Primary Standard and Buffer, ≥99.9% (titration), crystalline
Quality Level 300 description aminopeptidase substrate Assay ≥99.9% (titration) form crystalline storage condition dry at room temperature technique(s) ELISA: suitable protein extraction: suitable color white pH 10.5-12 useful pH range 7-9 pKa (25 °C) 8.1 bp 219-220 °C/10 mmHg (lit.) mp 167-172 °C (lit.) solubility methanol: soluble 26 mg/mL at 25 °C ethylene glycol: soluble 79.1 mg/mL at 25 °C water: soluble (678 g/l at 20 °C) absorption ≤0.05 at 290 nm at 40% suitability suitable for Western blot suitable for electrophoresis application(s) cell analysis diagnostic assay manufacturing life science and biopharma SMILES string NC(CO)(CO)CO InChI 1S/C4H11NO3/c5-4(1-6,2-7)3-8/h6-8H,1-3,5H2 InChI key LENZDBCJOHFCAS-UHFFFAOYSA-N DESCRIPTION General description Tris(hydroxymethyl)aminomethane, commonly known as Trometamol, Tris base, or Trizma® base, serves a pivotal role in diverse research applications as a biological buffer. Its optimal pKa of 8.1 makes it a preferred choice for formulating buffers like Tris-acetate-EDTA (TAE) and Tris-borate-EDTA (TBE), ensuring the maintenance of pH within the physiological range (pH 7 - 9), applicable to a wide spectrum of living organisms. Despite its utility, researchers need to exercise caution in protein studies, as Tris has the potential to interfere with the activity of specific enzymes. Tris base may find application as basimetric standard, independently as a buffer and as a crucial component in mixed buffer formulations, including Tris-EDTA (TE) buffer, TAE buffer, TBE buffer, among others. Its attributes include purity, essential stability, and a relative non-hygroscopic nature, making it a dependable choice in laboratory settings. In these environments, Tris base is indispensable for preparing buffers compatible with biological fluids and serves as a standard pH solution. It facilitates various laboratory procedures such as lactate dehydrogenase assays, in situ hybridization, and protein extraction from cells. The versatility of Tris base extends to cell biology, biochemistry, and protein research contributing significantly to studies involving cell membrane permeability and buffer preparation. Application Trizma® base has been used: - as a component of H buffer (cell dissociation buffer)[1] - for washing and saturation of wells in double sandwich ELISA immunoenzymatic technique[2] - as an assay buffer for reconstitution of extracted and dried protein samples[3] - to prepare Tris-HCl buffer that is used to stabilize proteins[4] - as a buffer to extract carotenoid from tubers[5] - as a component of sample buffer during protein extraction prior to western blotting[6] - as a component of sample buffer for sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE)[7] - to prepare simulated body fluid (SBF) for calcium phosphate (CaP) resorption assay[8] - as a buffer for polydopamine (PDA) deposition on stainless steel (SS) substrate[9] Features and Benefits Efficient buffering within the pH range of 7 - 9 with a pKa of 8.1 (25 °C) Tested to confirm low levels of heavy metal contamination, ensuring suitability for various applications Can be used in Cell Biology, and Biochemical research Other Notes 2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research For additional information on our range of Biochemicals, please complete this form. The pH values of all buffers are temperature- and concentration-dependent. For Tris buffers, pH increases about 0.03 unit per °C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution. For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode. Legal Information Trizma is a registered trademark of Merck KGaA, Darmstadt, Germany |
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