Sigma-Aldrich T3253 Trizma® hydrochloride reagent grade, ≥99.0% (titration), crystalline 250 gr
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Stok Kodu
LB.SA.T3253-250G
Kısa Bilgi
Synonym(s): TRIS HCl, TRIS hydrochloride, Tris(hydroxymethyl)aminomethane hydrochloride, Tromethane hydrochloride Linear Formula: NH2C(CH2OH)3 · HCl CAS Number: 1185-53-1 Molecular Weight: 157.60 Beilstein: 3675235 EC Number: 214-684-5 MDL number: MFCD00012590 PubChem Substance ID: 24900119 NACRES: NA.25
| Sigma-Aldrich T3253 Trizma® hydrochloride reagent grade, ≥99.0% (titration) |
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Synonym(s): TRIS HCl, TRIS hydrochloride, Tris(hydroxymethyl)aminomethane hydrochloride, Tromethane hydrochloride
Linear Formula: NH2C(CH2OH)3 · HCl
CAS Number: 1185-53-1
Molecular Weight: 157.60
Beilstein: 3675235
EC Number: 214-684-5
MDL number: MFCD00012590
PubChem Substance ID: 24900119
NACRES: NA.25
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PROPERTIES
grade reagent grade
Quality Level 400
assay ≥99.0% (titration)
form crystalline
impurities ≤0.5% water (Karl Fischer)
useful pH range 7.0-9.0
pKa (25 °C) 8.1
mp 150-152 °C
solubility H2O: soluble, clear, colorless
absorption ≤0.05 at 290 nm at 40%
application(s) diagnostic assay manufacturing
SMILES string Cl.NC(CO)(CO)CO
InChI 1S/C4H11NO3.ClH/c5-4(1-6,2-7)3-8;/h6-8H,1-3,5H2;1H
InChI key QKNYBSVHEMOAJP-UHFFFAOYSA-N
DESCRIPTION
General description
Trizma® hydrochloride is mainly required for preparation of buffer at physiological range of 7.3 to 7.5. The prepared buffer is compatible with biological fluids. It is of importance to laboratories as a standard pH solution.[1]
Application
The pH values of all buffers are temperature and concentration dependent. For Tris buffers, pH increases about 0.03 unit per °C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution. For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode. Trizma® hydrochloride has been used as a buffer solution for staining procedure, reduction and alkylation studies and in situ hybridization procedure.[2][3][4]
Packaging
100, 250, 500 g in poly bottle
1 kg in poly bottle
5, 10, 25, 50 kg in poly drum
Other Notes
Easily compare specifications for Trizma HCl products with the Trizma HCl specification table.
Legal Information
Trizma is a registered trademark of Sigma-Aldrich Co. LLC
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