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Sigma-Aldrich T3253 Trizma® hydrochloride reagent grade, ≥99.0% (titration), crystalline 500 gr

Stok Kodu
LB.SA.T3253-500G
Kısa Bilgi
Synonym(s): TRIS HCl, TRIS hydrochloride, Tris(hydroxymethyl)aminomethane hydrochloride, Tromethane hydrochloride Linear Formula: NH2C(CH2OH)3 · HCl CAS Number: 1185-53-1 Molecular Weight: 157.60 Beilstein: 3675235 EC Number: 214-684-5 MDL number: MFCD00012590 PubChem Substance ID: 24900119 NACRES: NA.25
Sigma-Aldrich T3253 Trizma® hydrochloride reagent grade, ≥99.0% (titration)
Synonym(s): TRIS HCl, TRIS hydrochloride, Tris(hydroxymethyl)aminomethane hydrochloride, Tromethane hydrochloride
Linear Formula: NH2C(CH2OH)3 · HCl
CAS Number: 1185-53-1
Molecular Weight: 157.60
Beilstein: 3675235
EC Number: 214-684-5
MDL number: MFCD00012590
PubChem Substance ID: 24900119
NACRES: NA.25
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Sigma-Aldrich T3253 Trizma® hydrochloride reagent grade, ≥99.0% (titration) 
PROPERTIES
grade reagent grade 
Quality Level 400 
assay ≥99.0% (titration) 
form crystalline 
impurities ≤0.5% water (Karl Fischer) 
useful pH range 7.0-9.0 
pKa (25 °C) 8.1 
mp 150-152 °C 
solubility H2O: soluble, clear, colorless 
absorption ≤0.05 at 290 nm at 40% 
application(s) diagnostic assay manufacturing 
SMILES string Cl.NC(CO)(CO)CO 
InChI 1S/C4H11NO3.ClH/c5-4(1-6,2-7)3-8;/h6-8H,1-3,5H2;1H 
InChI key QKNYBSVHEMOAJP-UHFFFAOYSA-N
 
DESCRIPTION
General description
Trizma® hydrochloride is mainly required for preparation of buffer at physiological range of 7.3 to 7.5. The prepared buffer is compatible with biological fluids. It is of importance to laboratories as a standard pH solution.[1]
Application
The pH values of all buffers are temperature and concentration dependent. For Tris buffers, pH increases about 0.03 unit per °C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution. For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode. Trizma® hydrochloride has been used as a buffer solution for staining procedure, reduction and alkylation studies and in situ hybridization procedure.­[2][3][4]
Packaging
100, 250, 500 g in poly bottle
1 kg in poly bottle
5, 10, 25, 50 kg in poly drum
Other Notes
Easily compare specifications for Trizma HCl products with the Trizma HCl specification table.
Legal Information
Trizma is a registered trademark of Sigma-Aldrich Co. LLC
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Sigma-Aldrich T3253 Trizma® hydrochloride reagent grade, ≥99.0% (titration), crystalline 500 gr Synonym(s): Tris(hydroxymethyl)aminomethane hydrochloride, Tromethane hydrochloride Linear Formula: NH2C(CH2OH)3 · HCl CAS Number: 1185-53-1 LB.SA.T3253-500G
Sigma-Aldrich T3253 Trizma® hydrochloride reagent grade, ≥99.0% (titration), crystalline 500 gr

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