Sigma-Aldrich T3824 Tyrosinase from mushroom lyophilized powder, ≥1000 unit/mg solid 250000 Units
Marka
Stok Kodu
LB.SA.T3824-250KU
Kısa Bilgi
Synonym(s): Catechol Oxidase, Monophenol Monooxygenase, Monophenol, dihydroxyphenylalanine:oxygen oxidoreductase, Polyphenol Oxidase CAS Number: 9002-10-2 Enzyme Commission number: 1.14.18.1 (BRENDA, IUBMB) MDL number: MFCD00082118 NACRES: NA.54
Bilgi
CAS Number: 9002-10-2
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Sigma-Aldrich T3824 Tyrosinase from mushroom lyophilized powder, ≥1000 unit/mg solid 250000 Units
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Synonym(s): Catechol Oxidase, Monophenol Monooxygenase, Monophenol, dihydroxyphenylalanine:oxygen oxidoreductase, Polyphenol Oxidase
CAS Number: 9002-10-2
Enzyme Commission number: 1.14.18.1 (BRENDA, IUBMB)
MDL number: MFCD00082118
NACRES: NA.54
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PROPERTIES
biological source fungus (mushroom)
Quality Level 200
form lyophilized powder
specific activity ≥1000 unit/mg solid
mol wt
119.5 kDa by electrophoresis
128 kDa by sedimentation velocity diffusion
133 kDa by LS
application(s) diagnostic assay manufacturing
shipped in wet ice
storage temp. −20°C
DESCRIPTION
General description
Isoelectric point (pI): 4.7-5
pH optimum is 6-7
Molecular weight: 128 kDa by sedimentation velocity diffusion; 133 kDa by light-scattering measurements, and 119.5 kDa by electrophoresis.
Tyrosinase is a copper-containing oxidase, which has activity for both catechols and cresol. It is responsible for browning reactions. The enzyme is reported to have two binding sites for aromatic substrates and a different binding site for oxygen-copper. The copper is probably Cu(I), with inactivation involving oxidation to Cu(II) ion. Tyrosinase is a copper-containing oxidase with activity for catechols and cresol. Tyrosinase participates in the conversion of tyrosine to dihydroxyphenylalanine (DOPA), and polymerization of DOPA leads to melanin formation. Tyrosinase has a role in browning reactions. The enzyme is said to have two binding sites for aromatic substrates and a distinct binding site for oxygen-copper.
Application
Tyrosinase activity has been assessed in a study that developed an alternative therapeutic agent for treating hyperpigmentation. [1] Tyrosinase has also been used in a study to investigate the oculocutaneous albinism phenotype in the Pakistani population. [2]
Packaging
25000, 50000 units in glass bottle
250000, 500000, 1000000 units in poly bottle
Biochem/physiol Actions
Converts tyrosine to L-DOPA or tyramine to dopamine
Physical properties
Isoelectric point (pI): 4.7-5
pH optimum is 6-7
Molecular weight: 128 kDa by sedimentation velocity diffusion; 133 kDa by light-scattering measurements, and 119.5 kDa by electrophoresis.
Unit Definition One
unit = ΔA280 of 0.001 per min at pH 6.5 at 25 °C in 3 mL reaction mix containing L-tyrosine.
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