Sigma-Aldrich T4415 Tris-Borate-EDTA buffer BioReagent, suitable for electrophoresis, 10× concentrate 4 L
Marka
Stok Kodu
LB.SA.T4415-4L
Kısa Bilgi
Synonym(s): TBE buffer MDL number: MFCD00236358 PubChem Substance ID: 329826793 NACRES: NA.25
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Sigma-Aldrich T4415 Tris-Borate-EDTA buffer BioReagent, suitable for electrophoresis |
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Synonym(s): TBE buffer
MDL number: MFCD00236358
PubChem Substance ID: 329826793
NACRES: NA.25 |
|
PROPERTIES
Quality Level 200
product line BioReagent
form solution
impurities DNase and RNase, none detected
suitability suitable for electrophoresis
SMILES string OB(O)O.NC(CO)(CO)CO.OC(=O)CN(CCN(CC(O)=O)CC(O)=O)CC(O)=O
InChI 1S/C10H16N2O8.C4H11NO3.BH3O3/c13-7(14)3-11(4-8(15)16)1-2-12(5-9(17)18)6-10(19)20;5-4(1-6,2-7)3-8;2-1(3)4/h1-6H2,(H,13,14)(H,15,16)(H,17,18)(H,19,20);6-8H,1-3,5H2;2-4H
InChI key OSBLTNPMIGYQGY-UHFFFAOYSA-N
DESCRIPTION
General description
TBE (Tris/Borate/EDTA) Buffer is commonly used in nucleic acid electrophoresis. This solution is effective under slightly basic conditions, which keeps DNA deprotonated, water-soluble, and protected from degradation. This concentrate can be easily diluted to 1x or 0.5x before use (with molecular biology grade water).[1][2]
Application
Ready for use in gel electrophoresis after dilution to working concentrations. Tris-Borate-EDTA buffer has been used for pulsed-field gel electrophoresis (PFGE).[3] TBE running buffer is the most commonly used buffer for DNA and RNA polyacrylamide gel electrophoresis. TBE is used with non-denaturing or denaturing (7 M urea) gels. It is also routinely used for DNA automated sequencing gel.
TBE can also be used for agarose gels but is not recommended for preparative gels for recovery of nucleic acids. Dilution of the TBE stock concentrates to a 1× TBE running buffer results in a buffer containing 89 mM Tris-borate and 2 mM EDTA, pH 8.3. The 5× or 10× stocks may also be added to an acrylamide/bis-acrylamide stock solution for making the PAGE gel. Applied voltages of less than 5 V/cm (distance between the electrodes of the unit) are recommended for maximum resolution.
Packaging
100 mL
1, 4, 10, 20, 200 L The 4L, 10L and 20L sizes are supplied in dispenser with a spigot.
Preparation Note
Prepared with 18 megohm water Prepared with Biotechnology Performance Certified Trizma base (Product Code T6066) and Molecular Biology Reagents boric acid (Product Code B6768) and EDTA disodium salt (Product Code E5134).
Other Notes
TBE buffer is prone to precipitation over time. Precipitation generally will not adversely affect performance. |
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