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Sigma-Aldrich T8154 Trypan Blue solution 0.4%, liquid, sterile-filtered, suitable for cell culture 100 mL

Sigma-Aldrich T8154 Trypan Blue solution 0.4%, liquid, sterile-filtered, suitable for cell culture 100 mL
Synonym(s): Blue cell viability dye
Empirical Formula (Hill Notation): C34H24N6Na4O14S4
CAS Number: 72-57-1
Molecular Weight: 960.81
Beilstein: 4360496
MDL number: MFCD00003969
PubChem Substance ID: 57654704
NACRES: NA.75
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Sigma-Aldrich T8154 Trypan Blue solution 0.4%, liquid, sterile-filtered, suitable for cell culture 20 mL
PROPERTIES
sterility sterile-filtered 
Quality Level 200 
form liquid 
storage condition dry at room temperature 
concentration 0.4% 
technique(s) cell culture | mammalian: suitable, tissue processing: suitable 
application(s) cell analysis 
shipped in ambient 
SMILES string [Na+].[Na+].[Na+].[Na+].Cc1cc(ccc1N=Nc2c(O)c3c(N)cc(cc3cc2S([O-])(=O)=O)S([O-])(=O)=O)-c4ccc(N=Nc5c(O)c6c(N)cc(cc6cc5S([O-])(=O)=O)S([O-])(=O)=O)c(C)c4 
InChI 1S/C34H28N6O14S4.4Na/c1-15-7-17(3-5-25(15)37-39-31-27(57(49,50)51)11-19-9-21(55(43,44)45)13-23(35)29(19)33(31)41)18-4-6-26(16(2)8-18)38-40-32-28(58(52,53)54)12-20-10-22(56(46,47)48)14-24(36)30(20)34(32)42;;;;/h3-14,41-42H,35-36H2,1-2H3,(H,43,44,45)(H,46,47,48)(H,49,50,51)(H,52,53,54);;;;/q;4*+1/p-4 
InChI key GLNADSQYFUSGOU-UHFFFAOYSA-J
 
DESCRIPTION
General description
Trypan Blue (TB), an anionic hydrophilic azo dye, crosses only the cell membranes of dead cells, thereby staining dead tissues/cells blue. It is used in quantitative microscopy for a dye exclusion staining technique to differentiate between live and dead mammalian cells. Trypan blue is a dye used to distinguish between live and dead cells. It is a vital stain that is not absorbed by healthy viable cells, but stains cells with a damaged cell membrane. This way, only dead cells can be counted. The method is sometimes referred to as the dye exclusion method.
Application
Trypan Blue solution has been used:
in cell viability assay to count viable and dead cells in rescue assay to count viable cells
in trypan blue exclusion counting method to determine proliferation curve
as the saline control and in the preparation of lipopolysaccharide (LPS) solution to confirm the success of LPS infusion
in trypan blue dye exclusion test to determine gastric epithelial cells (GEC) viability
to count GEC cells using a hemocytometer
Preparation Note
Prepared in 0.81% sodium chloride and 0.06% potassium phosphate, dibasic.
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Sigma-Aldrich T8154 Trypan Blue solution 0.4%, liquid, sterile-filtered, suitable for cell culture 100 mL Synonym(s): Blue cell viability dye, Empirical Formula (Hill Notation): C34H24N6Na4O14S4, CAS Number: 72-57-1, Molecular Weight: 960.81, Beilstein: 4360496 LB.SA.T8154-100ML
Sigma-Aldrich T8154 Trypan Blue solution 0.4%, liquid, sterile-filtered, suitable for cell culture 100 mL

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