Sigma-Aldrich T8154 Trypan Blue solution 0.4%, liquid, sterile-filtered, suitable for cell culture 20 mL
Marka
Stok Kodu
LB.SA.T8154-20ML
| Sigma-Aldrich T8154 Trypan Blue solution 0.4%, liquid, sterile-filtered, suitable for cell culture 20 mL |
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Synonym(s): Blue cell viability dye
Empirical Formula (Hill Notation): C34H24N6Na4O14S4
CAS Number: 72-57-1
Molecular Weight: 960.81
Beilstein: 4360496
MDL number: MFCD00003969
PubChem Substance ID: 57654704
NACRES: NA.75
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PROPERTIES
sterility sterile-filtered
Quality Level 200
form liquid
storage condition dry at room temperature
concentration 0.4%
technique(s) cell culture | mammalian: suitable, tissue processing: suitable
application(s) cell analysis
shipped in ambient
SMILES string [Na+].[Na+].[Na+].[Na+].Cc1cc(ccc1N=Nc2c(O)c3c(N)cc(cc3cc2S([O-])(=O)=O)S([O-])(=O)=O)-c4ccc(N=Nc5c(O)c6c(N)cc(cc6cc5S([O-])(=O)=O)S([O-])(=O)=O)c(C)c4
InChI 1S/C34H28N6O14S4.4Na/c1-15-7-17(3-5-25(15)37-39-31-27(57(49,50)51)11-19-9-21(55(43,44)45)13-23(35)29(19)33(31)41)18-4-6-26(16(2)8-18)38-40-32-28(58(52,53)54)12-20-10-22(56(46,47)48)14-24(36)30(20)34(32)42;;;;/h3-14,41-42H,35-36H2,1-2H3,(H,43,44,45)(H,46,47,48)(H,49,50,51)(H,52,53,54);;;;/q;4*+1/p-4
InChI key GLNADSQYFUSGOU-UHFFFAOYSA-J
DESCRIPTION
General description
Trypan Blue (TB), an anionic hydrophilic azo dye, crosses only the cell membranes of dead cells, thereby staining dead tissues/cells blue. It is used in quantitative microscopy for a dye exclusion staining technique to differentiate between live and dead mammalian cells. Trypan blue is a dye used to distinguish between live and dead cells. It is a vital stain that is not absorbed by healthy viable cells, but stains cells with a damaged cell membrane. This way, only dead cells can be counted. The method is sometimes referred to as the dye exclusion method.
Application
Trypan Blue solution has been used:
in cell viability assay to count viable and dead cells in rescue assay to count viable cells
in trypan blue exclusion counting method to determine proliferation curve
as the saline control and in the preparation of lipopolysaccharide (LPS) solution to confirm the success of LPS infusion
in trypan blue dye exclusion test to determine gastric epithelial cells (GEC) viability
to count GEC cells using a hemocytometer
Preparation Note
Prepared in 0.81% sodium chloride and 0.06% potassium phosphate, dibasic. |
