Sigma-Aldrich X1875 Xanthine Oxidase from bovine milk Grade I, ammonium sulfate suspension, ≥0.4 units/mg protein 5 UNITS
Marka
Stok Kodu
LB.SA.X1875-5UN
| Sigma-Aldrich X1875 Xanthine Oxidase from bovine milk Grade I, ammonium sulfate suspension, ≥0.4 units/mg protein 5 UNITS |
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Synonym(s): XOD, Xanthine:oxygen oxidoreductase
CAS Number: 9002-17-9
Enzyme Commission number: 1.17.3.2.
EC Number: 232-657-6
MDL number: MFCD00082145
NACRES: NA.54
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PROPERTIES
type Grade I
Quality Level 200
form ammonium sulfate suspension
specific activity ≥0.4 units/mg protein
foreign activity uricase ≤0.5%
shipped in wet ice
storage temp. 2-8 °C
DESCRIPTION
General description
Xanthine Oxidase (XOD) is a metal flavoprotein. It has flavin adenine dinucleotide (FAD), molybdenum and iron in the ratio 2:2:8. This homodimer has a molecule weight of 290kDa. It is a member of the molybdenum-protein family. This enzyme consists of two separated substrate-binding sites.
Formerly E.C. 1.1.3.22
Application
Xanthine Oxidase from bovine milk has been used:
in the preparation of xanthine oxidase (XO) solution for 5-(diethoxyphosphoryl)-5-methyl-1-pyrroline-N-oxide (DEPMPO)-spin trapping assay
in in vitro XO assay for screening Vietnamese medicinal plants for XO inhibitory activity
as a standard to determine XO activity
as a standard to test the synergistic effect of docosahexaenoic acid (DHA)
Packaging
5, 25 units in serum bottle
Biochem/physiol Actions
Hydroxylation of hypoxanthine to xanthine and xanthine to uric acid is catalyzed by xanthinebb oxidase (XO) enzyme. Xanthine oxidase is a molybdenum-containing enzyme that is found in the cytosol, and may be strongly inhibited by flavonoids. It plays a vital role in the metabolism of some drugs, as well as purines and pyrimidines. It is also known to be a biological source of reactive oxygen species. Xanthine oxidase was shown to be involved in the reduction of cytochrome c by the generation of superoxide anions following the oxidation of xanthine. These free radicals are responsible for reducing cytochrome c.
Unit Definition
One unit will convert 1.0 μmole of xanthine to uric acid per min at pH 7.5 at 25 °C. Approx. 50% of the activity is obtained with hypoxanthine as substrate.
Physical form
Suspension in 2.3 M (NH4)2SO4 containing 1 mM sodium salicylate
Analysis Note
Protein determined by biuret |