Sigma-Aldrich X4126 Xylene Cyanol FF for molecular biology, BioReagent 10 gr
Marka
Stok Kodu
LB.SA.X4126-10G
Kısa Bilgi
Synonym(s): Acid blue 147, Cyanol FF, XC Empirical Formula (Hill Notation): C25H27N2NaO6S2 CAS Number: 2650-17-1 Molecular Weight: 538.61 Colour Index Number: 42135 EC Number: 220-167-5 MDL number: MFCD00019481 PubChem Substance ID: 57654732 NACRES: NA.52
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Sigma-Aldrich X4126 Xylene Cyanol FF for molecular biology, BioReagent 10 gr |
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Synonym(s): Acid blue 147, Cyanol FF, XC
Empirical Formula (Hill Notation): C25H27N2NaO6S2
CAS Number: 2650-17-1
Molecular Weight: 538.61
Colour Index Number: 42135
EC Number: 220-167-5
MDL number: MFCD00019481
PubChem Substance ID: 57654732
NACRES: NA.52
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PROPERTIES
grade for molecular biology
Quality Level 200
product line BioReagent
form powder
solubility H2O: complete 1 mg/mL, clear, blue
storage temp. room temp
SMILES string [Na+].CCNc1ccc(cc1C)C(=C2/C=CC(=N\CC)\C(C)=C2)\c3ccc(cc3S(O)(=O)=O)S([O-])(=O)=O
InChI 1S/C25H28N2O6S2.Na/c1-5-26-22-11-7-18(13-16(22)3)25(19-8-12-23(27-6-2)17(4)14-19)21-10-9-20(34(28,29)30)15-24(21)35(31,32)33;/h7-15,26H,5-6H2,1-4H3,(H,28,29,30)(H,31,32,33);/q;+1/p-1/b25-19-,27-23-; InChI key VVLFAAMTGMGYBS-KRQUPCAFSA-M
DESCRIPTION
General description
Xylene cyanol is often used as a tracking dye during agarose or polyacrylamide gel electrophoresis. It has a slight negative charge and will migrate the same direction as DNA, allowing the user to monitor the progress of molecules moving through the gel. The rate of migration varies with gel composition.
Application
Xylene Cyanol FF is used as a:
histological stain and as tracker dye in DNA sequencing and electrophoresis[1][2]
gel-loading dye[3]
formamide RNA loading dye[4]
tracking dye in native polyacrylamide gel electrophoresis (PAGE) loading buffer[5]
Packaging
10 g in glass bottle
Biochem/physiol Actions
Xylene Cyanol FF acts as nucleic acid stain.
Reconstitution
A 6x Gel Loading Buffer can be made using standard recipes, including 0.05% xylene cyanol. |
