Sınırlı Stok
ZYMO RESEARCH D5017 Methylated & Non-methylated pUC19 DNA Set 20 ng
Kategori
Marka
Stok Kodu
LB.MB.ZYMD5017
Kısa Bilgi
Ideal control for cytosine modification studies including HPLC; LC/MS; TLC
| ZYMO RESEARCH D5017 Methylated & Non-methylated pUC19 DNA Set 20 ng |
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Includes pUC19MN primers in 20 µl |
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The Methylated & Non-methylated pUC19 DNA Set consists of control DNAs and a set of specifically designed primers. The set is ideal as a "spike-in” control to assess bisulfite conversion efficiency within the same reaction as the sample, or to produce known mixtures of methylated and non-methylated DNA for assay calibration. The non-methylated pUC19 DNA is pUC19 isolated from a methylation-negative strain of bacteria (Dam–, Dcm–), and the methylated pUC19 DNA is pUC19 enzymatically methylated at all cytosines in the dinucleotide sequence 5′... CpG... 3′ by CpG Methylase.
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