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ZYMO RESEARCH D5043 EZ-96 DNA Methylation-Gold MagPrep 8 x 96 Rxns.
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ZYMO RESEARCH D5043 EZ-96 DNA Methylation-Gold MagPrep 8 x 96 Rxns. |
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Complete, high-throughput bisulfite conversion of GC-rich DNA in less than 3 hours.
A coupled heat denaturation/conversion reaction step streamlines the conversion of non-methylated cytosines to uracil.
High throughput (96-well), automated desulphonation and recovery of bisulfite-treated DNA.
Eluted, ultra-pure DNA is ideal for use in subsequent molecular-based analyses. |
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The EZ-96 DNA Methylation-Gold MagPrep integrates DNA denaturation and bisulfite conversion processes into one step followed by a magnetic bead-based clean-up for high-throughput methylation analysis. To streamline the procedure, high temperature is used instead of sodium hydroxide to denature DNA. Desulphonation and clean-up of the converted DNA is performed while bound to the MagBinding Beads. The EZ DNA Methylation-Gold kits have been designed to minimize template degradation, loss of DNA during treatment and clean-up, and to provide complete conversion of unmethylated cytosines. Recovered DNA is ideal for downstream analyses, including PCR amplification, endonuclease digestion, sequencing, microarrays, etc.
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