Sınırlı Stok
ZYMO RESEARCH D5451 RRHP 5-hmC Library Prep Kit 25 Preps
| ZYMO RESEARCH D5451 RRHP 5-hmC Library Prep Kit 25 Preps |
|
Innovative library preparation for strand-specific mapping of 5-hmC in DNA
Streamlined workflow accommodates low (≥100ng) DNA inputs
Libraries are ready for Next-Generation sequencing (Illumina-compatible) |
|
The RRHP 5-hmC Library Prep Kit is an all-inclusive solution for analysis of genome-wide 5-hydroxymethylcytosine (5-hmC) positions at single-base resolution. The Reduced Representation Hydroxymethylation Profiling (RRHP) method is based on blocking MspI digestion by glucosylating 5-hmC within MspI recognition sites. Fragments lacking glucosylated 5-hmC at the adapter-ligation junction will be cleaved and not amplified by PCR. Therefore, only fragments containing 5-hmC will be successfully amplified and analyzed by Next-Generation Sequencing. Fragments with higher 5-hmC levels will be correlated with higher frequency of sequencing reads. RRHP bypasses the need for bisulfite conversion, which allows for DNA inputs as low as 100 ng, lower sequencing depth, and straight-forward bioinformatics processing.
|
