BOSTER R01709 Recombinant Human FGF10 Protein 100 μg/vial
| R01709 Recombinant Human FGF10 Protein 100 μg/vial |
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Cat. No: R01709
Size: 100 μg
Amino Acid Sequence:AAIGS CSKEY RVLLG QLQKQTDLMQDTSRLLDPYI RIQGL DVPKL REHCRERPGAFPSEETLRGL GRRGF LQTLN ATLGCVLHRLADLEQRLPKA QDLER SGLNI EDLEK LQMARPNILGLRNNI YCMAQ LLDNS DTAEP TKAGRGASQPPTPTP ASDAF QRKLE GCRFL HGYHRFMHSVGRVFS KWGES PNRSR
Source: E. coli Gln38-Ser208
Species: human
Purity: >95%, by SDS-PAGEquantitativedensitometry by Coomassie® Blue Staining
Molecular Weight: 19.4KD
Formulation: Lyophilized after extensivedialysis against PBS
Reconstitution: Reconstitute in ddH2Oat 100μg/mL
Endotoxin: Less than 1 EU/μg of rHubFGF10as determined by LAL method
Storage:Lyophilized recombinant human KGF-2/FGF10remains stable up to 6 months at -80 °C from date of receipt. Upon reconstitution, KGF-2/FGF10 remains stable up to 2 weeks at
4 °C or up to 3 months at -20 °C
Description
The Fibroblast Growth Factors (FGFs) are heparin binding glycoproteins that exert a variety of biological activities toward cells of mesenchymal, neuronal, and epithelial origin. FGF-10 belongs to the subgroup of FGFs that also includes FGF-3, -7, and -22 (1). Mature human FGF-10 is an approximately 20 kDa protein that contains a serine-rich region near its N-terminus FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. This protein exhibits mitogenic activity for keratinizing epidermal cells, but essentially no activity for fibroblasts, which is similar to the biological activity of FGF7. Studies of the mouse homolog of suggested that this gene is required for embryonic epidermal morphogenesis including brain development, lung morphogenesis, and initiation of limb bud formation. This gene is also implicated to be a primary factor in the process of wound healing. |
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A Recombinant protein is a protein produced artificially by an expression host organism, aka expression system, that is transfected with a recombinant gene (target gene) isolated from another organism. The purpose is to produce the target gene encoded protein in large quantities for medical, research and academic uses. Expression Systems There are several common expression systems for producing recombinant proteins, such as E. Coli, Yeast, Insect Cell lines such as Sf21, mammalian cells such as CHO, HEK, and human cell lines. These expression systems act as the factory for recombinant protein production, where the cellular mechanisms involved in protein production uses the recombinant gene in the vector as blueprint to mass produce the target protein. E. Coli is the first organism to be used as an expression system. It is easy to use however cannot produce protein with post-translational modification (PTM) because it lacks the cellular mechanisms of eukaryotic cells which is necessary for PTM of proteins. Thus for producing simple proteins and for applications that do not require proteins to have PTM, E. Coli is a sufficient and convenient expression system however for more complex applications, more advanced expression systems are required. Expression Vectors A vector is a tool for manipulating DNA. It is the transport vehicle for the gene of the protein of interest. There are many types of vectors, some common vectors are plasmids and reverse transcription viruses. The DNA that encodes the protein is first synthesized in vitro, or cloned from native host DNA templates, then inserted into the vector DNA. The host organism is processed to take up vectors. These vectors, once inside the corresponding host cells, will be transcribed and translated into proteins. |
