Sigma Aldrich 114391 Bromophenol Blue ACS reagent 25 gr
Kategori
Marka
Stok Kodu
LB.SA.114391-25G
Kısa Bilgi
Synonym(s): 3′,3″,5′,5″-Tetrabromophenolsulfonphthalein, Bromphenol Blue Sultone Form Empirical Formula (Hill Notation): C19H10Br4O5S CAS Number: 115-39-9 Molecular Weight: 669.96 Beilstein: 61698 EC Number: 204-086-2 MDL number: MFCD00005875 PubChem Substance ID: 24847169 NACRES: NA.47
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Sigma Aldrich 114391 Bromophenol Blue ACS reagent 25 gr |
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Synonym(s): 3′,3″,5′,5″-Tetrabromophenolsulfonphthalein, Bromphenol Blue Sultone Form
Empirical Formula (Hill Notation): C19H10Br4O5S
CAS Number: 115-39-9
Molecular Weight: 669.96
Beilstein: 61698
EC Number: 204-086-2
MDL number: MFCD00005875
PubChem Substance ID: 24847169
NACRES: NA.47 |
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PROPERTIES
grade ACS reagent
Quality Level 200
form powder
technique(s) titration: suitable
visual transition interval 3.0-4.6, yellow to blue
mp 273 °C (lit.)
solubility ethanol: 0.1%
application(s) diagnostic assay manufacturing, hematology, histology
storage temp. room temp
SMILES string Oc1c(Br)cc(cc1Br)C2(OS(=O)(=O)c3ccccc23)c4cc(Br)c(O)c(Br)c4
InChI 1S/C19H10Br4O5S/c20-12-5-9(6-13(21)17(12)24)19(10-7-14(22)18(25)15(23)8-10)11-3-1-2-4-16(11)29(26,27)28-19/h1-8,24-25H
InChI key UDSAIICHUKSCKT-UHFFFAOYSA-N
DESCRIPTION
General description
Bromophenol blue is present as a sulfonated hydroxyquinone in acidic aqueous solutions. It is also known as tetrabromophenolsulfonphthalein.Bromophenol Blue is a pH indicator and a dye appearing as a strong blue color. It has a slight negative charge and will migrate the same direction as DNA, allowing the user to monitor the progress of molecules moving through the gel. The rate of migration varies with gel composition.
Application
Bromphenol Blue is used as a tracking dye in DNA, RNA (agarose), and protein (polyacrylamide) gel electrophoresis. It has been used in the preparation of protein samples for western blotting analysis. Bromophenol blue migrates at approximately the same rate as 300-500bp DNA in agarose gel and at the buffer front in protein polyacrylamide gels. It has also been known for its use as a vital stain to probe the blood-brain barrier. |
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