Sigma Aldrich 114413 Bromophenol Blue ACS reagent, Dye content 95 % 5 gr
Marka
Stok Kodu
LB.SA.114413-5G
Kısa Bilgi
Synonym(s): 3′,3″-Dibromothymolsulfonphthalein Empirical Formula (Hill Notation): C27H28Br2O5S CAS Number: 76-59-5 Molecular Weight: 624.38 Beilstein: 373934 EC Number: 200-971-2 MDL number: MFCD00005872 PubChem Substance ID: 24847171
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Sigma Aldrich 114413 Bromophenol Blue ACS reagent, Dye content 95 % 5 gr |
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Synonym(s): 3′,3″-Dibromothymolsulfonphthalein
Empirical Formula (Hill Notation): C27H28Br2O5S
CAS Number: 76-59-5
Molecular Weight: 624.38
Beilstein: 373934
EC Number: 200-971-2
MDL number: MFCD00005872
PubChem Substance ID: 24847171 |
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PROPERTIES
grade ACS reagent
Quality Level 200
form powder
composition Dye content, 95%
visual transition interval 6.0-7.6, yellow to blue(passes test)
mp 200-202 °C (lit.)
λmax 420 nm
ε (extinction coefficient)
≥17000 at 419-425 nm in methanol
≥8000 at 326-332 nm in methanol
≥9000 at 279-285 nm in methanol
storage temp. room temp
SMILES string CC(C)c1cc(c(C)c(Br)c1O)C2(OS(=O)(=O)c3ccccc23)c4cc(C(C)C)c(O)c(Br)c4C
InChI 1S/C27H28Br2O5S/c1-13(2)17-11-20(15(5)23(28)25(17)30)27(19-9-7-8-10-22(19)35(32,33)34-27)21-12-18(14(3)4)26(31)24(29)16(21)6/h7-14,30-31H,1-6H3
InChI key NUHCTOLBWMJMLX-UHFFFAOYSA-N
DESCRIPTION
General description
Bromothymol Blue is a sulfonated hydroxyquinone in acidic aqueous solutions. In alkaline solutions, this dye is present as a moderately sized dianion. It is also known as bromthymol blue and dibromothymolsulfonphthalein.
Application
Bromothymol blue dye serves as a pH indicator because of the precise color transition at neutrality. Other applications include:
- as a vital stain to trace the movement of fluids from the lymph and to define cell walls or nuclei under the microscope
- for the demonstration of fungal hyphae within plant roots
- in the assessment of the soluble acidity within single rice grains
- use in an agar gel medium for enumeration of Bacillus cereus
- as a component of several bacterial growth and detection substrates such as cystine lactose electrolyte deficient (CLED/BROLACIN); polymyxin pyruvate egg yolk mannitol bromothymol blue (PEMBA) and MacConkey agar. |
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