Sigma-Aldrich A8097 Acridine Orange hydrochloride solution 10 mg/mL in H2O 10 mL
Kategori
Marka
Stok Kodu
LB.SA.A8097-10ML
Kısa Bilgi
Synonym(s): 3,6-Bis(dimethylamino)acridine hydrochloride Empirical Formula (Hill Notation): C17H19N3 · HCl CAS Number: 65-61-2 Molecular Weight: 301.81 Beilstein: 3575587 MDL number: MFCD00012660 PubChem Substance ID: 24891317 NACRES: NA.47
Garanti Süresi
24 Ay
| Sigma-Aldrich A8097 Acridine Orange hydrochloride solution 10 mg/mL in H2O 10 mL |
|
Synonym(s): 3,6-Bis(dimethylamino)acridine hydrochloride
Empirical Formula (Hill Notation): C17H19N3 · HCl
CAS Number: 65-61-2
Molecular Weight: 301.81
Beilstein: 3575587
MDL number: MFCD00012660
PubChem Substance ID: 24891317
NACRES: NA.47 |
|
PROPERTIES
Assay >95.0% (HPLC)
Quality Level 200
form solution
concentration 10 mg/mL in H2O
color orange to red
εmax 47-54 at 486-492 nm in water
application(s)
diagnostic assay manufacturing
hematology
histology
storage temp. room temp
SMILES string Cl[H].CN(C)c1ccc2cc3ccc(cc3nc2c1)N(C)C
InChI 1S/C17H19N3.ClH/c1-19(2)14-7-5-12-9-13-6-8-15(20(3)4)11-17(13)18-16(12)10-14;/h5-11H,1-4H3;1H
InChI key VSTHNGLPHBTRMB-UHFFFAOYSA-N
DESCRIPTION
Application
A rigorously purified zinc-free material suitable for use in flow cytometry. Acridine Orange hydrochloride hydrate dye has been used:
- to stain intracellular acidic vesicles.[1]
- to measure lysosomal membrane permeability (LMP).[1]
- or autophagy detection.[2]
Fluorescent stain for nucleic acids in agarose and polyacrylamide gels. Has been used to study apoptosis in various cell culture and tissue models.
Biochem/physiol Actions
Acridine Orange is a metachromatic dye which can stain DNA, RNA and acid glycosaminoglycans. At low concentrations, it intercalates into DNA and precipitates RNA. However, at high concentrations it denatures and precipitates both RNA and DNA.[3] Acridine Orange is also used to analyze autophagy. It enters into acidic organelles in a pH-dependent manner. At neutral pH, acridine orange gives a green fluorescence and in acidic conditions, it accumulates in acidic organelles giving a bright red fluorescence.[4]
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