Sigma-Aldrich C3389 Acetylcholinesterase from Electrophorus electricus (electric eel) Type VI-S, lyophilized powder, 200-1,000 units/mg protein 10000 Units
Marka
Stok Kodu
LB.SA.C3389-10KU
Kısa Bilgi
Synonym(s): AChE, Acetylcholine acetylhydrolase, Cholinesterase, Acetyl CAS Number: 9000-81-1 Enzyme Commission number: 3.1.1.7 (BRENDA, IUBMB) EC Number: 232-559-3 MDL number: MFCD00081268
Bilgi
CAS Number: 9000-81-1
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Sigma-Aldrich C3389 Acetylcholinesterase from Electrophorus electricus (electric eel) Type VI-S, lyophilized powder, 200-1,000 units/mg protein
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| Synonym(s): AChE, Acetylcholine acetylhydrolase, Cholinesterase, Acetyl CAS Number: 9000-81-1 Enzyme Commission number: 3.1.1.7 (BRENDA, IUBMB) EC Number: 232-559-3 MDL number: MFCD00081268 |
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PROPERTIES
type Type VI-S
Quality Level 200 form lyophilized powder specific activity 200-1,000 units/mg protein mol wt 280 kDa composition Protein, ≥45% biuret solubility 20 mM Tris HCl buffer, pH 7.5: soluble 1.0 mg/mL, clear(lit.) application(s) diagnostic assay manufacturing storage temp. −20°C DESCRIPTION
General description
Molecular Weight: 280 kDa Isoelectric Point: 5.5 Extinction Coefficient: E1% = 18.0 (280 nm) Acetylcholinesterase from Electrophorus electricus is a tetramer composed of 4 equal subunits of 70 kDa each. Each subunit contains one active site. The enzyme is a glycoprotein containing hexosamines. Application The enzyme from sigma has been used as a reference to to evaluate the effect of aspartame metabolites on hippocampal acetylcholinesterase activity.[1] The enzyme has also been used in immobilization studies for the rapid detection of acetylthiocholine chloride.[2] Biochem/physiol Actions Major degradative enzyme for acetylcholine in vivo. Converts acetylcholine + H2O to choline + acetic acid. Unit Definition One unit will hydrolyze 1.0 μmole of acetylcholine to choline and acetate per min at pH 8.0 at 37 °C. Physical form Lyophilized powder containing Tris buffer salts Preparation Note This enzyme dissolves in 20 mM Tris-HCl buffer, pH 7.5 at 1 mg/mL concentration, yielding a clear solution. Analysis Note The activity obtained using acetylcholine as substrate is 30-100 times that obtained with butyrylcholine, using acetylcholinesterase from electric eel. |
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