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Sigma-Aldrich C3389 Acetylcholinesterase from Electrophorus electricus (electric eel) Type VI-S, lyophilized powder, 200-1,000 units/mg protein 500 Units

Stok Kodu
LB.SA.C3389-500UN
Kısa Bilgi
Synonym(s): AChE, Acetylcholine acetylhydrolase, Cholinesterase, Acetyl CAS Number: 9000-81-1 Enzyme Commission number: 3.1.1.7 (BRENDA, IUBMB) EC Number: 232-559-3 MDL number: MFCD00081268
Bilgi
CAS Number: 9000-81-1
Ön Siparişli Ürün
Sigma-Aldrich C3389 Acetylcholinesterase from Electrophorus electricus (electric eel) Type VI-S, lyophilized powder, 200-1,000 units/mg protein
Synonym(s): AChE, Acetylcholine acetylhydrolase, Cholinesterase, Acetyl
CAS Number:
9000-81-1
Enzyme Commission number:
3.1.1.7 (BRENDA, IUBMB)
EC Number:
232-559-3
MDL number:
MFCD00081268
≥99%  
PROPERTIES
type Type VI-S
Quality Level
200
form
lyophilized powder
specific activity
200-1,000 units/mg protein
mol wt
280 kDa
composition
Protein, ≥45% biuret
solubility
20 mM Tris HCl buffer, pH 7.5: soluble 1.0 mg/mL, clear(lit.)
application(s)
diagnostic assay manufacturing
storage temp.
−20°C
 
DESCRIPTION
General description
Molecular Weight: 280 kDa
Isoelectric Point: 5.5
Extinction Coefficient: E1% = 18.0 (280 nm)
Acetylcholinesterase from Electrophorus electricus is a tetramer composed of 4 equal subunits of 70 kDa each. Each subunit contains one active site. The enzyme is a glycoprotein containing hexosamines.
Application
The enzyme from sigma has been used as a reference to to evaluate the effect of aspartame metabolites on hippocampal acetylcholinesterase activity.[1] The enzyme has also been used in immobilization studies for the rapid detection of acetylthiocholine chloride.[2]
Biochem/physiol Actions
Major degradative enzyme for acetylcholine in vivo. Converts acetylcholine + H2O to choline + acetic acid.
Unit Definition
One unit will hydrolyze 1.0 μmole of acetylcholine to choline and acetate per min at pH 8.0 at 37 °C. Physical form Lyophilized powder containing Tris buffer salts
Preparation Note
This enzyme dissolves in 20 mM Tris-HCl buffer, pH 7.5 at 1 mg/mL concentration, yielding a clear solution.
Analysis Note
The activity obtained using acetylcholine as substrate is 30-100 times that obtained with butyrylcholine, using acetylcholinesterase from electric eel.
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Sigma-Aldrich F0127 D-(−)-Fructose ≥99%
Sigma-Aldrich C3389 Acetylcholinesterase from Electrophorus electricus (electric eel) Type VI-S, lyophilized powder, 200-1,000 units/mg protein 500 Units Synonym(s): AChE, Acetylcholine acetylhydrolase, Cholinesterase, Acetyl CAS Number: 9000-81-1 Enzyme Commission number: 3.1.1.7 (BRENDA, IUBMB) LB.SA.C3389-500UN
Sigma-Aldrich C3389 Acetylcholinesterase from Electrophorus electricus (electric eel) Type VI-S, lyophilized powder, 200-1,000 units/mg protein 500 Units

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