Sigma-Aldrich M5655 Thiazolyl Blue Tetrazolium Bromide powder, BioReagent, suitable for cell culture, suitable for insect cell culture, ≥97.5% (HPLC) 500 mg
Marka
Stok Kodu
LB.SA.M5655-500MG
Kısa Bilgi
Synonym(s): 3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide, MTT, Methylthiazolyldiphenyl-tetrazolium bromide Empirical Formula (Hill Notation): C18H16BrN5S CAS Number: 298-93-1 Molecular Weight: 414.32 Beilstein: 4081397 EC Number: 206-069-5 MDL number: MFCD00011964 PubChem Substance ID: 24897030 NACRES: NA.75
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Sigma-Aldrich M5655 Thiazolyl Blue Tetrazolium Bromide powder, BioReagent, suitable for cell culture, suitable for insect cell culture, ≥97.5% (HPLC) |
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Synonym(s): 3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide, MTT, Methylthiazolyldiphenyl-tetrazolium bromide
Empirical Formula (Hill Notation): C18H16BrN5S
CAS Number: 298-93-1
Molecular Weight: 414.32
Beilstein: 4081397
EC Number: 206-069-5
MDL number: MFCD00011964
PubChem Substance ID: 24897030
NACRES: NA.75 |
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PROPERTIES
product line BioReagent
Quality Level 200
Assay ≥97.5% (HPLC)
form powder
technique(s) cell culture | insect: suitable, cell culture | mammalian: suitable
mp 195 °C (dec.) (lit.)
solubility H2O: 5 mg/mL
storage temp. 2-8°C
SMILES string [Br-].Cc1nc(sc1C)-[n+]2nc(nn2-c3ccccc3)-c4ccccc4
InChI 1S/C18H16N5S.BrH/c1-13-14(2)24-18(19-13)23-21-17(15-9-5-3-6-10-15)20-22(23)16-11-7-4-8-12-16;/h3-12H,1-2H3;1H/q+1;/p-1
InChI key AZKSAVLVSZKNRD-UHFFFAOYSA-M
DESCRIPTION
Application
Thiazolyl Blue Tetrazolium Blue (MTT) may be used in measurement of cell proliferation. MTT produces a yellowish solution that is converted to dark blue, water-insoluble MTT formazan by mitochondrial dehydrogenases of living cells. The blue crystals are solubilized with acidified isopropanol and the intensity is measured colorimetrically at 570 nm. MTT has been used as a histochemical/cytochemical reagent and for the detection of NAD. ADP-linked enzyme systems in tissue cannot be detected with MTT, due to binding of the cation by the cyanide trap used. MTT is rapidly reduced to the formazan, which chelates with nickel, copper, and cobalt; the cobalt chelate has been used in oxidative systems. Thiazolyl Blue Tetrazolium Bromide has been used as a colorimetric metabolic activity indicator in cell viability assays (MTT assay).[1][2][3][4] It has also been used to determine cell proliferation.[5] |
Tavsiye Ürünler
3.246,88 TL + KDV
3.896,26 TL
