Sigma-Aldrich MABT825 Anti-beta-Actin Antibody, clone 4C2 clone 4C2, from mouse 100 µL
Marka
Stok Kodu
LB.SA.MABT825-100UL
Kısa Bilgi
Synonym(s): Actin, cytoplasmic 1, beta-Actin eCl@ss: 32160702 NACRES: NA.41
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Sigma-Aldrich MABT825 Anti-beta-Actin Antibody, clone 4C2 clone 4C2, from mouse |
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Synonym(s): Actin, cytoplasmic 1, beta-Actin
eCl@ss: 32160702
NACRES: NA.41 |
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PROPERTIES
biological source mouse
Quality Level 100
antibody form purified immunoglobulin
antibody product type primary antibodies
clone 4C2, monoclonal
species reactivity chicken, human, mouse, rat
species reactivity (predicted by homology) mammals (based on 100% sequence homology)
technique(s)
immunocytochemistry: suitable
western blot: suitable
isotype IgG1κ
NCBI accession no. NP_001092.1
UniProt accession no. P60709
shipped in wet ice
Gene Information human ... ACTB(60)
DESCRIPTION
General description
Actin, cytoplasmic 1 (UniProt P60709; also known as Beta-actin) is encoded by the ACTB gene (Gene ID 60) in human. Actins are globular multi-functional proteins that serve as the basic building blocks of cytoskeletal microfilaments and are among the most conserved eukaryotic proteins. Six actin types exist, skeletal muscle alpha-actin is encoded by the ACTA1 gene, smooth muscle alpha-actin by the ACTA2 gene, cytoplasmic beta-actin by the ACTB gene, cardiac muscle alpha-actin by the ACTC1 gene, cytoplasmic gamma-actin by the ACTG1 gene, and smooth muscle gamma-actin by the ACTG2 (a.k.a. ACTA3) gene. Although actins show >90% overall sequence homology, isoforms do show spatial, temporal, and tissue-specific expression patterns and only 50-60% homology is found in their 18 N-terminal residues. Cytoplasmic β and γ-actins, are thought to be present in all cells, while the other four actin isoforms are typically found in specific adult muscle tissue types. Actins exist in a variety of structural states, depending on the specific ionic conditions or the interaction with ligand proteins. The oligomeric and polymeric forms that actin molecules assume are dependent on the distinct conformations they adopt.
Specificity
Clone 4C2 detected BSA conjugated with beta-actin N-terminal peptide, but not BSA conjugated with N-terminal peptides derived from the 5 other actin types (Dugina, V., et al. (2009). J. Cell Sci. 122(Pt 16):2980-2988).
Immunogen
KLH-conjugated linear peptide corresponding to the N-terminal sequence of human beta-Actin. |
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