Sigma-Aldrich 07-606 Anti-phospho-JAK2 (Tyr1007/1008) Antibody Upstate®, from rabbit
Marka
Stok Kodu
LB.SA.07-606
Kısa Bilgi
Synonym(s): Janus kinase 2, Janus kinase 2 (a protein tyrosine kinase), tyrosine-protein kinase JAK2 eCl@ss: 32160702 NACRES: NA.41
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Sigma-Aldrich 07-606 Anti-phospho-JAK2 (Tyr1007/1008) Antibody Upstate®, from rabbit |
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Synonym(s): Janus kinase 2, Janus kinase 2 (a protein tyrosine kinase), tyrosine-protein kinase JAK2
eCl@ss: 32160702
NACRES: NA.41 |
|
PROPERTIES
biological source rabbit
Quality Level 100
antibody form purified immunoglobulin
antibody product type primary antibodies
clone polyclonal
species reactivity rat, mouse, human
manufacturer/tradename Upstate®
technique(s) western blot: suitable
isotype IgG
NCBI accession no. NP_004963
UniProt accession no. O60674
shipped in wet ice
Gene Information human ... JAK2(3717), mouse ... Jak2(16452), rat ... Jak2(24514)
DESCRIPTION
General description
JAK2 (Janus Kinase 2) belongs to the emerging family of non-receptor Janus tyrosine kinases, which regulate a spectrum of cellular functions downstream of activated cytokine receptors in the lympho-hematopoietic system. Immunological stimuli, such as interferons and cytokines, induce recruitment of Stat transcription factors to cytokine receptor-associated JAK2. JAK2 then phosphorylates proximal Stat factors, which subsequently dimerize, translocate to the nucleus and bind to CIS elements upstream of target gene promoters to regulate transcription. The canonical JAK/Stat pathway is integral to maintaining a normal immune system by stimulating proliferation, differentiation, survival and host resistance to pathogens. Altering JAK/Stat signaling to reduce cytokine induced pro-inflammatory responses represents an attractive target for anti-inflammatory therapies. Within the JAK2 kinase domain, there is a region that has considerable sequence homology to the regulatory region of the insulin receptor. Among a variety of sites, Tyrosines 1007 and 1008 are sites of trans- or autophosphorylation in vivo and in in vitro kinase reactions.
Specificity
Predicted to cross-react with rat based on sequence homology.
Recognizes phosphorylated JAK2, Mr 130 kDa.
Immunogen
Epitope: Tyr1007/1008
KLH-conjugated, synthetic peptide corresponding to amino acids surrounding phosphotyrosines 1007 and 1008 of human JAK2.
Application
Anti-phospho-JAK2 (Tyr1007/1008) Antibody detects level of phospho-JAK2 (Tyr1007/1008) & has been published & validated for use in WB.
Research Category
Signaling
Research Sub Category
Immunological Signaling
Quality
Routinely evaluated by Western Blot on Pervanadate treated L6 lysates.
Western Blot Analysis: 1:500 dilution of this lot detected PHOS-JAK2 (TYR1007/1008) on 10 ug of Pervanadate treated L6 lysates.
Target description
130 kDa Linkage Replaces: 04-1098
Linkage Replaces: 04-1098
Physical form
Format: Purified
Protein A purified
Purified rabbit IgG in buffer containing 0.1M Tris-Glycine, 0.15M NaCl, 0.05% Sodium Azide, pH7.4.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Analysis Note
Control Human ovarian cancer cells, GM-CSF-treated TF-1 cells.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
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