Sigma-Aldrich SRP3157 Sox2 human recombinant, expressed in E. coli, ≥95% (SDS-PAGE), ≥95% (HPLC), suitable for cell culture 25 μG
Marka
Stok Kodu
LB.SA.SRP3157-25UG
| Sigma-Aldrich SRP3157 Sox2 human recombinant, expressed in E. coli, ≥95% (SDS-PAGE), ≥95% (HPLC), suitable for cell culture 25 μg |
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NACRES: NA.32
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PROPERTIES
biological source human
recombinant expressed in E. coli
assay ≥95% (HPLC), ≥95% (SDS-PAGE)
form lyophilized
mol wt 34.3 kDa
packaging pkg of 25 μg
technique(s) cell culture | mammalian: suitable
impurities <0.1 EU/μg endotoxin, tested
color white
UniProt accession no. P48431
DESCRIPTION
General description
Sox2 (sex determining region Y (SRY)-box 2) is one of the 20 human SOX transcription factors, which are characterized by the highly conserved ∼80-residues DNA-binding HMG (high-mobility group) domain. This domain was initially identified in the testis-determining factor Sry.[1] Recombinant human Sox2 is a 34.3 kDa protein containing 317 amino-acid residues.
Biochem/physiol Actions
Sox2 (sex determining region Y (SRY)-box 2) transcription factor participates in maintaining self-renewal and pluripotency of embryonic stem cells. The expression of this protein is aberrant in various human malignancies, and it acts as an oncogene in esophageal squamous cell carcinoma (SCC).[2] SOX2 promotes proilferation, migration and adhesion abilities of dental pulp stem cells (DPSCs), and this might have applications in tissue engineering.[3] It participates in Ewing′s sarcoma cell proliferation, and its inactivation results in apoptosis and G1/S arrest, in a PI3K (phosphoinositide 3-kinase)/Akt pathway-mediated manner.[4]
Sequence
MYNMMETELK PPGPQQTSGG GGGNSTAAAA GGNQKNSPDR VKRPMNAFMV WSRGQRRKMA QENPKMHNSE ISKRLGAEWK LLSETEKRPF IDEAKRLRAL HMKEHPDYKY RPRRKTKTLM KKDKYTLPGG LLAPGGNSMA SGVGVGAGLG AGVNQRMDSY AHMNGWSNGS YSMMQDQLGY PQHPGLNAHG AAQMQPMHRY DVSALQYNSM TSSQTYMNGS PTYSMSYSQQ GTPGMALGSM GSVVKSEASS SPPVVTSSSH SRAPCQAGDL RDMISMYLPG AEVPEPAAPS RLHMSQHYQS GPVPGTAING TLPLSHM
Physical form
Lyophilized from 10 mM Sodium Acetate, pH 6.0.
Reconstitution
Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1- 1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C. |
