Sigma-Aldrich SRP3182 VEGF human Animal-component free, recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC), suitable for cell culture 10 μg
Marka
Stok Kodu
LB.SA.SRP3182-10UG
Kısa Bilgi
Synonym(s): Folliculostellate cell-derived growth factor, Glioma-derived endothelial cell mitogen, VPF, Vascular Endothelial Growth Factor-A NACRES: NA.32
| Sigma-Aldrich SRP3182 VEGF human Animal-component free, recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC), suitable for cell culture 10 μg |
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Synonym(s): Folliculostellate cell-derived growth factor, Glioma-derived endothelial cell mitogen, VPF, Vascular Endothelial Growth Factor-A
NACRES: NA.32
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PROPERTIES
biological source human
recombinant expressed in E. coli
assay ≥98% (HPLC), ≥98% (SDS-PAGE)
form lyophilized
potency 1.0-8.0 ng/mL
mol wt 38.2 kDa
packaging pkg of 10 μg
technique(s) cell culture | mammalian: suitable
impurities <0.1 EU/μg endotoxin, tested
color white
UniProt accession no. P15692
shipped in wet ice
storage temp. −20°C
Gene Information human ... VEGFA(7422)
DESCRIPTION
General description
VEGF (vascular endothelial growth factor) signals through the three receptors; fms-like tyrosine kinase (flt-1), KDR gene product (the murine homolog of KDR is the flk-1 gene product) and the flt4 gene product.[1] Recombinant human VEGF is a 38.2kDa disulfide-linked homodimeric protein consisting of two 165 amino acid polypeptide chains.
Application
VEGF human has been used as a chemoattractant for HUVECs (human umbilical vein endothelial cells) migration assay.[2]
Packaging
10 μg
Biochem/physiol
Actions VEGF (vascular endothelial growth factor) is a potent growth and angiogenic cytokine. It stimulates proliferation and survival of endothelial cells, and promotes angiogenesis and vascular permeability. Expressed in vascularized tissues, VEGF plays a prominent role in normal and pathological angiogenesis.[3] Substantial evidence implicates VEGF in the induction of tumor metastasis and intraocular neovascular syndromes.[4][5] Polymorphism in VEGF at C(-634)G is linked with diabetic retinopathy in type 2 diabetes.[6]
Sequence
APMAEGGGQN HHEVVKFMDV YQRSYCHPIE TLVDIFQEYP DEIEYIFKPS CVPLMRCGGC CNDEGLECVP TEESNITMQI MRIKPHQGQH IGEMSFLQHN KCECRPKKDR ARQENPCGPC SERRKHLFVQ DPQTCKCSCK NTDSRCKARQ LELNERTCRC DKPRR
Physical form
Lyophilized with no additives.
Reconstitution
Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a stabilizer (example 5% Trehalose) and store in working aliquots at -20°C to -80°C. |
