Sigma Aldrich Y2377 2x YT medium Powder microbial growth medium 250 gr
Marka
Stok Kodu
LB.SA.Y2377-250G
Kısa Bilgi
Synonym(s): 2x Yeast Extract Tryptone medium, 2xTY medium, 2xYT Medium, 2xYT media UNSPSC Code: 41106225 NACRES: NA.85
| Sigma Aldrich Y2377 2x YT medium Powder microbial growth medium 250 gr |
| Synonym(s): 2x Yeast Extract Tryptone medium, 2xTY medium, 2xYT Medium, 2xYT media UNSPSC Code: 41106225 NACRES: NA.85 |
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PROPERTIES
grade for molecular biology
Quality Level 200 sterility non-sterile form powder technique(s) microbiological culture: suitable pH 6.8-7.2(3.1% solution) application(s) food and beverages, microbiology storage temp. room temp suitability nonselective for Escherichia coli, nonselective for coliforms DESCRIPTION General description 2xYT (2x Yeast Extract Tryptone medium) is a standard microbial growth medium used for the cultivation of E. coli and M13 bacteriophage. This nutrient-rich microbial broth contains peptides, amino acids, and water-soluble vitamins in a low-salt formulation. Application 2x YT medium has been used for culturing E. coli BL21 for cloning [1], E. coli SS320 cells for scaffold synthesis [2], and NEB 5-alpha competent E. coli cells for protospacer adjacent motif (PAM) binding assays. It has also been used in the culturing of Agrobacterium tumefaciens[3] and E. coli DH10B.[4] Features and Benefits 2xYT powder provides: - Standard formulation - An inexpensive alternative to liquid Components 16 g/L Tryptone 10 g/L Yeast Extract 5 g/L NaCl Preparation Note 1. Stir to suspend 31 g of 2xYT Microbial Medium Powder in 1 L of water. 2. Heat to boiling while stirring to dissolve all ingredients completely. 3. Autoclave for 15 minutes at 121 °C. Reconstitution Add 31 g powder in 1L water. Heat to boiling while stirring to dissolve powder. Autoclave for 15 minutes at 121°C to sterilize. Allow to cool before making additions, such as antibiotics (if desired). |
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